Sometimes considered as the most effective variant, the Model 52 was developed to again shorten the wings to increase speed and dispense with the folding wing mechanism. In addition, ailerons, aileron trim tab and flaps were revised. Produced first by Mitsubishi, most Model 52s were made by Nakajima. The prototype was made in June 1943 by modifying an A6M3 and was first flown in August 1943. The first Model 52 is said in the handling manual to have 製造番号 (seizō bangō, or production number) 3904, which apparently refers to the prototype.
Research by Mr. Bunzo Komine published by Mr. Kenji Miyazaki states that aircraft 3904 through 4103 had the same exhaust system and cowl flaps as on the Model 22. This is partially corroborated by two wrecks researched by Mr. Stan Gajda and Mr. L. G. Halls, production number 4007 and 4043, respectively. (The upper cowling was slightly redesigned from that of the Model 22.)